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aec tlgt immortalized cells  (Innovative Cell Technologies Inc)

 
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    Innovative Cell Technologies Inc aec tlgt immortalized cells
    Schematic illustration of 3D co‐culture setup <t>using</t> <t>AEC‐tLgT</t> epithelial cells and MLg mouse fibroblasts. Epithelial cells and fibroblasts are mixed with Matrigel and loaded onto Transwell filters and provided nutrients through medium perfusion from the outside chamber. GFR, growth factor‐reduced; PET, polyester.
    Aec Tlgt Immortalized Cells, supplied by Innovative Cell Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aec+tlgt+immortalized+cells/pmc13130368-203-37-54?v=Innovative+Cell+Technologies+Inc
    Average 86 stars, based on 1 article reviews
    aec tlgt immortalized cells - by Bioz Stars, 2026-08
    86/100 stars

    Images

    1) Product Images from "Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung"

    Article Title: Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung

    Journal: Current Protocols

    doi: 10.1002/cpz1.70372

    Schematic illustration of 3D co‐culture setup using AEC‐tLgT epithelial cells and MLg mouse fibroblasts. Epithelial cells and fibroblasts are mixed with Matrigel and loaded onto Transwell filters and provided nutrients through medium perfusion from the outside chamber. GFR, growth factor‐reduced; PET, polyester.
    Figure Legend Snippet: Schematic illustration of 3D co‐culture setup using AEC‐tLgT epithelial cells and MLg mouse fibroblasts. Epithelial cells and fibroblasts are mixed with Matrigel and loaded onto Transwell filters and provided nutrients through medium perfusion from the outside chamber. GFR, growth factor‐reduced; PET, polyester.

    Techniques Used: Co-Culture Assay

    Representative brightfield and fluorescent images of GFP + /tdTomato + double‐positive AEC‐tLgT cells after FACS sorting and expansion. Images were taken at 10× magnification on an ECHO Revolve R4 fluorescence microscope. Scale bar, 100 µm.
    Figure Legend Snippet: Representative brightfield and fluorescent images of GFP + /tdTomato + double‐positive AEC‐tLgT cells after FACS sorting and expansion. Images were taken at 10× magnification on an ECHO Revolve R4 fluorescence microscope. Scale bar, 100 µm.

    Techniques Used: Fluorescence, Microscopy

    Representative brightfield images of MLg fibroblasts at 50% confluence, the optimal growth pattern to set up 3D co‐cultures with AEC‐tLgT cells. Images taken at 4× magnification (left; scale bar, 900 µm) and 10× magnification (right; scale bar, 360 µm) on an ECHO Revolve R4 fluorescence microscope.
    Figure Legend Snippet: Representative brightfield images of MLg fibroblasts at 50% confluence, the optimal growth pattern to set up 3D co‐cultures with AEC‐tLgT cells. Images taken at 4× magnification (left; scale bar, 900 µm) and 10× magnification (right; scale bar, 360 µm) on an ECHO Revolve R4 fluorescence microscope.

    Techniques Used: Fluorescence, Microscopy

    Representative images of AEC‐tLgT organoids after 2 months of growth. Large panel is a brightfield image of one Transwell taken at 4× magnification. Scale bar, 900 µm. Small panels were taken under both brightfield and fluorescence at 10× magnification. Scale bar, 100 µm. Images were taken on an ECHO Revolve R4 fluorescence microscope.
    Figure Legend Snippet: Representative images of AEC‐tLgT organoids after 2 months of growth. Large panel is a brightfield image of one Transwell taken at 4× magnification. Scale bar, 900 µm. Small panels were taken under both brightfield and fluorescence at 10× magnification. Scale bar, 100 µm. Images were taken on an ECHO Revolve R4 fluorescence microscope.

    Techniques Used: Fluorescence, Microscopy

    Representative data and images for CSC‐ and NiCl 2 ‐treated AEC‐tLgT cells. ( A ) RT‐qPCR was used to measure induction of xenobiotic response‐related genes CYP1A1 and CYP1B1 after 48 hr CSC treatment of AEC‐tLgT cells. mRNA abundance was normalized to actin ( ACTB ) levels and fold change was calculated over DMSO vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( B ) Representative brightfield images at 4× magnification of vehicle‐ and CSC‐treated samples showing progressive morphological changes with increasing CSC dose. ( C ) Representative RT‐qPCR results for vehicle‐ and nickel (NiCl 2 )‐treated AEC‐tLgT cells after 48 hr showing induction of metal response genes CA9 and NDRG1 . mRNA abundance was normalized to MTF1 levels and fold change was calculated over water vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( D ) Representative brightfield images at 4× magnification of vehicle‐ and NiCl 2 ‐treated samples showing stable cell survival across NiCl 2 .
    Figure Legend Snippet: Representative data and images for CSC‐ and NiCl 2 ‐treated AEC‐tLgT cells. ( A ) RT‐qPCR was used to measure induction of xenobiotic response‐related genes CYP1A1 and CYP1B1 after 48 hr CSC treatment of AEC‐tLgT cells. mRNA abundance was normalized to actin ( ACTB ) levels and fold change was calculated over DMSO vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( B ) Representative brightfield images at 4× magnification of vehicle‐ and CSC‐treated samples showing progressive morphological changes with increasing CSC dose. ( C ) Representative RT‐qPCR results for vehicle‐ and nickel (NiCl 2 )‐treated AEC‐tLgT cells after 48 hr showing induction of metal response genes CA9 and NDRG1 . mRNA abundance was normalized to MTF1 levels and fold change was calculated over water vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( D ) Representative brightfield images at 4× magnification of vehicle‐ and NiCl 2 ‐treated samples showing stable cell survival across NiCl 2 .

    Techniques Used: Quantitative RT-PCR, Stable Transfection



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    aec tlgt immortalized cells  (Innovative Cell Technologies Inc)
    86
    Innovative Cell Technologies Inc aec tlgt immortalized cells
    Schematic illustration of 3D co‐culture setup <t>using</t> <t>AEC‐tLgT</t> epithelial cells and MLg mouse fibroblasts. Epithelial cells and fibroblasts are mixed with Matrigel and loaded onto Transwell filters and provided nutrients through medium perfusion from the outside chamber. GFR, growth factor‐reduced; PET, polyester.
    Aec Tlgt Immortalized Cells, supplied by Innovative Cell Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aec+tlgt+immortalized+cells/pmc13130368-203-37-54?v=Innovative+Cell+Technologies+Inc
    Average 86 stars, based on 1 article reviews
    aec tlgt immortalized cells - by Bioz Stars, 2026-08
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Schematic illustration of 3D co‐culture setup using AEC‐tLgT epithelial cells and MLg mouse fibroblasts. Epithelial cells and fibroblasts are mixed with Matrigel and loaded onto Transwell filters and provided nutrients through medium perfusion from the outside chamber. GFR, growth factor‐reduced; PET, polyester.

    Journal: Current Protocols

    Article Title: Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung

    doi: 10.1002/cpz1.70372

    Figure Lengend Snippet: Schematic illustration of 3D co‐culture setup using AEC‐tLgT epithelial cells and MLg mouse fibroblasts. Epithelial cells and fibroblasts are mixed with Matrigel and loaded onto Transwell filters and provided nutrients through medium perfusion from the outside chamber. GFR, growth factor‐reduced; PET, polyester.

    Article Snippet: MLg mouse lung fibroblasts (ATCC, CCL‐206, RRID:CVCL_0437) MLF medium (MLg fibroblast culture medium, see recipe) Fmed+ROCKinh culture medium (see recipe) Sterile 1× DPBS (Corning, 21‐031‐CV) 0.05% (w/v) trypsin‐EDTA solution (Gibco, 25300‐062) 0.4% trypan blue staining (Gibco, 15250‐061) AEC‐tLgT immortalized cells (from Basic Protocol ) GFR Matrigel (Corning, 354230) 3D basic medium (see recipe) Accutase (Innovative Cell Technologies, AT‐104) SB‐431542, working stock of 1 mM (Selleck Chemicals, S1067) 15‐ and 50‐ml conical tubes Benchtop centrifuge that can accommodate 15‐ and 50‐ml conical tubes Hemocytometer (Thermo Fisher Scientific, 02‐671‐5) 15‐cm tissue culture dish (Corning, CLS430599) or T‐175 culture flask with vented cap T‐25 culture flask with vented cap 8‐ or 10‐in. length straight specimen forceps, serrated (e.g., VWR, 82027‐436 or ‐434) 24‐well Transwell inserts (Corning, 3470) 24‐well tissue culture plate 1.5‐ml microcentrifuge tubes Bucket or container of ice to bring into the tissue culture hood Sealed 200‐μl pipette filter tips, pre‐cooled at 4°C to be used in the hood (optional) Brightfield microscope with either 4× or 2.5× objective

    Techniques: Co-Culture Assay

    Representative brightfield and fluorescent images of GFP + /tdTomato + double‐positive AEC‐tLgT cells after FACS sorting and expansion. Images were taken at 10× magnification on an ECHO Revolve R4 fluorescence microscope. Scale bar, 100 µm.

    Journal: Current Protocols

    Article Title: Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung

    doi: 10.1002/cpz1.70372

    Figure Lengend Snippet: Representative brightfield and fluorescent images of GFP + /tdTomato + double‐positive AEC‐tLgT cells after FACS sorting and expansion. Images were taken at 10× magnification on an ECHO Revolve R4 fluorescence microscope. Scale bar, 100 µm.

    Article Snippet: MLg mouse lung fibroblasts (ATCC, CCL‐206, RRID:CVCL_0437) MLF medium (MLg fibroblast culture medium, see recipe) Fmed+ROCKinh culture medium (see recipe) Sterile 1× DPBS (Corning, 21‐031‐CV) 0.05% (w/v) trypsin‐EDTA solution (Gibco, 25300‐062) 0.4% trypan blue staining (Gibco, 15250‐061) AEC‐tLgT immortalized cells (from Basic Protocol ) GFR Matrigel (Corning, 354230) 3D basic medium (see recipe) Accutase (Innovative Cell Technologies, AT‐104) SB‐431542, working stock of 1 mM (Selleck Chemicals, S1067) 15‐ and 50‐ml conical tubes Benchtop centrifuge that can accommodate 15‐ and 50‐ml conical tubes Hemocytometer (Thermo Fisher Scientific, 02‐671‐5) 15‐cm tissue culture dish (Corning, CLS430599) or T‐175 culture flask with vented cap T‐25 culture flask with vented cap 8‐ or 10‐in. length straight specimen forceps, serrated (e.g., VWR, 82027‐436 or ‐434) 24‐well Transwell inserts (Corning, 3470) 24‐well tissue culture plate 1.5‐ml microcentrifuge tubes Bucket or container of ice to bring into the tissue culture hood Sealed 200‐μl pipette filter tips, pre‐cooled at 4°C to be used in the hood (optional) Brightfield microscope with either 4× or 2.5× objective

    Techniques: Fluorescence, Microscopy

    Representative brightfield images of MLg fibroblasts at 50% confluence, the optimal growth pattern to set up 3D co‐cultures with AEC‐tLgT cells. Images taken at 4× magnification (left; scale bar, 900 µm) and 10× magnification (right; scale bar, 360 µm) on an ECHO Revolve R4 fluorescence microscope.

    Journal: Current Protocols

    Article Title: Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung

    doi: 10.1002/cpz1.70372

    Figure Lengend Snippet: Representative brightfield images of MLg fibroblasts at 50% confluence, the optimal growth pattern to set up 3D co‐cultures with AEC‐tLgT cells. Images taken at 4× magnification (left; scale bar, 900 µm) and 10× magnification (right; scale bar, 360 µm) on an ECHO Revolve R4 fluorescence microscope.

    Article Snippet: MLg mouse lung fibroblasts (ATCC, CCL‐206, RRID:CVCL_0437) MLF medium (MLg fibroblast culture medium, see recipe) Fmed+ROCKinh culture medium (see recipe) Sterile 1× DPBS (Corning, 21‐031‐CV) 0.05% (w/v) trypsin‐EDTA solution (Gibco, 25300‐062) 0.4% trypan blue staining (Gibco, 15250‐061) AEC‐tLgT immortalized cells (from Basic Protocol ) GFR Matrigel (Corning, 354230) 3D basic medium (see recipe) Accutase (Innovative Cell Technologies, AT‐104) SB‐431542, working stock of 1 mM (Selleck Chemicals, S1067) 15‐ and 50‐ml conical tubes Benchtop centrifuge that can accommodate 15‐ and 50‐ml conical tubes Hemocytometer (Thermo Fisher Scientific, 02‐671‐5) 15‐cm tissue culture dish (Corning, CLS430599) or T‐175 culture flask with vented cap T‐25 culture flask with vented cap 8‐ or 10‐in. length straight specimen forceps, serrated (e.g., VWR, 82027‐436 or ‐434) 24‐well Transwell inserts (Corning, 3470) 24‐well tissue culture plate 1.5‐ml microcentrifuge tubes Bucket or container of ice to bring into the tissue culture hood Sealed 200‐μl pipette filter tips, pre‐cooled at 4°C to be used in the hood (optional) Brightfield microscope with either 4× or 2.5× objective

    Techniques: Fluorescence, Microscopy

    Representative images of AEC‐tLgT organoids after 2 months of growth. Large panel is a brightfield image of one Transwell taken at 4× magnification. Scale bar, 900 µm. Small panels were taken under both brightfield and fluorescence at 10× magnification. Scale bar, 100 µm. Images were taken on an ECHO Revolve R4 fluorescence microscope.

    Journal: Current Protocols

    Article Title: Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung

    doi: 10.1002/cpz1.70372

    Figure Lengend Snippet: Representative images of AEC‐tLgT organoids after 2 months of growth. Large panel is a brightfield image of one Transwell taken at 4× magnification. Scale bar, 900 µm. Small panels were taken under both brightfield and fluorescence at 10× magnification. Scale bar, 100 µm. Images were taken on an ECHO Revolve R4 fluorescence microscope.

    Article Snippet: MLg mouse lung fibroblasts (ATCC, CCL‐206, RRID:CVCL_0437) MLF medium (MLg fibroblast culture medium, see recipe) Fmed+ROCKinh culture medium (see recipe) Sterile 1× DPBS (Corning, 21‐031‐CV) 0.05% (w/v) trypsin‐EDTA solution (Gibco, 25300‐062) 0.4% trypan blue staining (Gibco, 15250‐061) AEC‐tLgT immortalized cells (from Basic Protocol ) GFR Matrigel (Corning, 354230) 3D basic medium (see recipe) Accutase (Innovative Cell Technologies, AT‐104) SB‐431542, working stock of 1 mM (Selleck Chemicals, S1067) 15‐ and 50‐ml conical tubes Benchtop centrifuge that can accommodate 15‐ and 50‐ml conical tubes Hemocytometer (Thermo Fisher Scientific, 02‐671‐5) 15‐cm tissue culture dish (Corning, CLS430599) or T‐175 culture flask with vented cap T‐25 culture flask with vented cap 8‐ or 10‐in. length straight specimen forceps, serrated (e.g., VWR, 82027‐436 or ‐434) 24‐well Transwell inserts (Corning, 3470) 24‐well tissue culture plate 1.5‐ml microcentrifuge tubes Bucket or container of ice to bring into the tissue culture hood Sealed 200‐μl pipette filter tips, pre‐cooled at 4°C to be used in the hood (optional) Brightfield microscope with either 4× or 2.5× objective

    Techniques: Fluorescence, Microscopy

    Representative data and images for CSC‐ and NiCl 2 ‐treated AEC‐tLgT cells. ( A ) RT‐qPCR was used to measure induction of xenobiotic response‐related genes CYP1A1 and CYP1B1 after 48 hr CSC treatment of AEC‐tLgT cells. mRNA abundance was normalized to actin ( ACTB ) levels and fold change was calculated over DMSO vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( B ) Representative brightfield images at 4× magnification of vehicle‐ and CSC‐treated samples showing progressive morphological changes with increasing CSC dose. ( C ) Representative RT‐qPCR results for vehicle‐ and nickel (NiCl 2 )‐treated AEC‐tLgT cells after 48 hr showing induction of metal response genes CA9 and NDRG1 . mRNA abundance was normalized to MTF1 levels and fold change was calculated over water vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( D ) Representative brightfield images at 4× magnification of vehicle‐ and NiCl 2 ‐treated samples showing stable cell survival across NiCl 2 .

    Journal: Current Protocols

    Article Title: Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal Lung

    doi: 10.1002/cpz1.70372

    Figure Lengend Snippet: Representative data and images for CSC‐ and NiCl 2 ‐treated AEC‐tLgT cells. ( A ) RT‐qPCR was used to measure induction of xenobiotic response‐related genes CYP1A1 and CYP1B1 after 48 hr CSC treatment of AEC‐tLgT cells. mRNA abundance was normalized to actin ( ACTB ) levels and fold change was calculated over DMSO vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( B ) Representative brightfield images at 4× magnification of vehicle‐ and CSC‐treated samples showing progressive morphological changes with increasing CSC dose. ( C ) Representative RT‐qPCR results for vehicle‐ and nickel (NiCl 2 )‐treated AEC‐tLgT cells after 48 hr showing induction of metal response genes CA9 and NDRG1 . mRNA abundance was normalized to MTF1 levels and fold change was calculated over water vehicle‐treated samples. N = 3. Values shown as mean ± SD . ( D ) Representative brightfield images at 4× magnification of vehicle‐ and NiCl 2 ‐treated samples showing stable cell survival across NiCl 2 .

    Article Snippet: MLg mouse lung fibroblasts (ATCC, CCL‐206, RRID:CVCL_0437) MLF medium (MLg fibroblast culture medium, see recipe) Fmed+ROCKinh culture medium (see recipe) Sterile 1× DPBS (Corning, 21‐031‐CV) 0.05% (w/v) trypsin‐EDTA solution (Gibco, 25300‐062) 0.4% trypan blue staining (Gibco, 15250‐061) AEC‐tLgT immortalized cells (from Basic Protocol ) GFR Matrigel (Corning, 354230) 3D basic medium (see recipe) Accutase (Innovative Cell Technologies, AT‐104) SB‐431542, working stock of 1 mM (Selleck Chemicals, S1067) 15‐ and 50‐ml conical tubes Benchtop centrifuge that can accommodate 15‐ and 50‐ml conical tubes Hemocytometer (Thermo Fisher Scientific, 02‐671‐5) 15‐cm tissue culture dish (Corning, CLS430599) or T‐175 culture flask with vented cap T‐25 culture flask with vented cap 8‐ or 10‐in. length straight specimen forceps, serrated (e.g., VWR, 82027‐436 or ‐434) 24‐well Transwell inserts (Corning, 3470) 24‐well tissue culture plate 1.5‐ml microcentrifuge tubes Bucket or container of ice to bring into the tissue culture hood Sealed 200‐μl pipette filter tips, pre‐cooled at 4°C to be used in the hood (optional) Brightfield microscope with either 4× or 2.5× objective

    Techniques: Quantitative RT-PCR, Stable Transfection